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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has become noticed in several tumors, which ends up in chromosome instability, and therefore may lead to tumorigenesis. This protein was uncovered to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a crucial job in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are already described. Two further splice variants have been described but detailed nucleotide sequence facts is not still obtainable.
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Blocking peptides are peptides that bind specifically towards the goal antibody and block antibody binding. These peptide typically is made up of the epitope regarded through the antibody.
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This guide outlines further doable optimization of cellular and immunoassay parameters to be sure the best possible results are...
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This information outlines even further doable optimization of mobile and immunoassay parameters to guarantee the very best effects are...
Antibodies bound to the blocking peptide not bind to your epitope to the focus on protein. By comparing the staining with the blocked antibody compared to the antibody by yourself, you can see which staining is specific.
Target Information Cyclin E belongs into the highly conserved cyclin family, whose users are characterised by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show distinctive expression and degradation styles which lead into the temporal coordination of every mitotic occasion. Cyclin E sorts a posh with and capabilities being a regulatory subunit of CDK2, whose action is required for cell cycle G1/S changeover. This protein accumulates within the G1-S section boundary and is particularly degraded as cells development by S stage.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both of those antibodies...
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